Treu Becky L, Minteer Shelley D
Department of Chemistry, Saint Louis University, 3501 Laclede Ave., St. Louis, MO 63103, USA.
Bioelectrochemistry. 2008 Nov;74(1):73-7. doi: 10.1016/j.bioelechem.2008.07.005. Epub 2008 Jul 24.
This research details the isolation and purification of a new type of lactate dehydrogenase that is dependent upon the coenzyme pyrroloquinoline quinone (PQQ). PQQ-dependent enzymes have been of interest in the literature over the last decade due to the fact that many of them can undergo direct electron transfer (DET) at electrode surfaces which is of interest for biosensor and biofuel cell applications. In the paper, we detail the isolation of PQQ-dependent lactate dehydrogenase (PQQ-LDH) from two sources of Gluconobacter (Gluconobacter sp. 33 and Gluconobacter suboxydans). This paper also shows the first evidence that PQQ-LDH can undergo direct electron transfer at gold and carbon electrode surfaces for future use in biosensors and biofuel cells.
本研究详细阐述了一种新型的依赖于辅酶吡咯喹啉醌（PQQ）的乳酸脱氢酶的分离与纯化。在过去十年中，依赖PQQ的酶一直受到文献关注，因为其中许多酶能够在电极表面进行直接电子转移（DET），这对于生物传感器和生物燃料电池应用具有重要意义。在本文中，我们详细描述了从两种葡糖杆菌来源（葡糖杆菌属33和氧化葡糖杆菌）中分离PQQ依赖性乳酸脱氢酶（PQQ-LDH）的过程。本文还首次证明了PQQ-LDH能够在金电极和碳电极表面进行直接电子转移，以便未来用于生物传感器和生物燃料电池。
