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葡萄糖酸杆菌在生物传感器中的应用:从葡萄糖酸杆菌和醋酸杆菌中分离出的全细胞和酶在生物传感器构建中的应用。

Gluconobacter in biosensors: applications of whole cells and enzymes isolated from Gluconobacter and Acetobacter to biosensor construction.

作者信息

Svitel Juraj, Tkác Jan, Vostiar Igor, Navrátil Marian, Stefuca Vladimír, Bucko Marek, Gemeiner Peter

机构信息

Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK 812 37, Bratislava, Slovakia.

出版信息

Biotechnol Lett. 2006 Dec;28(24):2003-10. doi: 10.1007/s10529-006-9195-3. Epub 2006 Oct 28.

Abstract

Bacteria belonging to the genus Acetobacter and Gluconobacter, and enzymes isolated from them, have been extensively used for biosensor construction in the last decade. Bacteria used as a biocatalyst are easy to prepare and use in amperometric biosensors. They contain multiple enzyme activities otherwise not available commercially. The range of compounds analyzable by Gluconobacter biosensors includes: mono- and poly-alcohols, multiple aldoses and ketoses, several disaccharides, triacylglycerols, and complex parameters like utilizable saccharides or biological O2 demand. Here, the recent trends in Gluconobacter biosensors and current practical applications are summarized.

摘要

在过去十年中,醋酸杆菌属和葡糖杆菌属细菌及其分离出的酶已被广泛用于构建生物传感器。用作生物催化剂的细菌易于制备且可用于电流型生物传感器。它们具有多种酶活性,而这些酶活性在商业上无法获得。葡糖杆菌生物传感器可分析的化合物范围包括:一元醇和多元醇、多种醛糖和酮糖、几种二糖、三酰甘油以及可利用糖类或生物需氧量等复杂参数。本文总结了葡糖杆菌生物传感器的最新趋势和当前的实际应用。

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