碲化镉@二氧化硅颗粒在免疫分析中的应用。

The application of CdTe@SiO2 particles in immunoassay.

作者信息

Jin Li, Yu Dong-Dong, Liu Yan, Zhao Xiao-Li, Zhou Jian-Guang

机构信息

Department of Analytical Chemistry, College of Chemistry, Jilin University, Jiefang Road 2519, Changchun 130023, China.

出版信息

Talanta. 2008 Sep 15;76(5):1053-7. doi: 10.1016/j.talanta.2008.05.002. Epub 2008 May 9.

DOI:10.1016/j.talanta.2008.05.002

PMID:18761154

Abstract

CdTe@SiO(2) fluorescent particles were synthesized via hydrolysis and condensation of tetraethyl orthosilicate (TEOS) in water-in-oil (W/O) emulsion. Uniform luminophore-doped silica nanoparticles with 100nm in diameter were obtained using microemulsion method and characterized by SEM. Antibody proteins were successfully conjugated to the fluorescent particles by the reaction of avidin and biotin, which were confirmed by fluorescence spectra. CdTe@SiO(2) fluorescent particles were potentially useful for the applications in biolabeling and imaging.

摘要

通过在油包水（W/O）乳液中对正硅酸四乙酯（TEOS）进行水解和缩合反应，合成了CdTe@SiO(2)荧光颗粒。采用微乳液法获得了直径为100nm的均匀发光体掺杂二氧化硅纳米颗粒，并通过扫描电子显微镜（SEM）对其进行了表征。通过抗生物素蛋白和生物素的反应，抗体蛋白成功地与荧光颗粒偶联，荧光光谱证实了这一点。CdTe@SiO(2)荧光颗粒在生物标记和成像应用中具有潜在用途。