Wang Chao, Ma Qiang, Dou Wenchao, Kanwal Shamsa, Wang Guannan, Yuan Pingfan, Su Xingguang
Department of Analytical Chemistry, College of Chemistry, Jilin University, 10 Qianwei Road, Changchun 130012, China.
Talanta. 2009 Feb 15;77(4):1358-64. doi: 10.1016/j.talanta.2008.09.018. Epub 2008 Sep 19.
This paper presents the synthesis of aqueous CdTe QDs embedded silica nanoparticles by reverse microemulsion method and their applications as fluorescence probes in bioassay and cell imaging. With the aim of embedding more CdTe QDs in silica spheres, we use poly(dimethyldiallyl ammonium chloride) to balance the electrostatic repulsion between CdTe QDs and silica intermediates. By modifying the surface of CdTe/SiO(2) composite nanoparticles with amino and methylphosphonate groups, biologically functionalized and monodisperse CdTe/SiO(2) composite nanoparticles can be obtained. In this work, CdTe/SiO(2) composite nanoparticles are conjugated with biotin-labeled mouse IgG via covalent binding. The biotin-labeled mouse IgG on the CdTe/SiO(2) composite nanoparticles surface can recognize FITC-labeled avidin and avidin on the surface of polystyrene microspheres by protein-protein binding. Finally, the CdTe/SiO(2) composite nanoparticles with secondary antibody are used to label the MG63 osteosarcoma cell with primary antibody successfully, which demonstrates that the application of CdTe/SiO(2) composite nanoparticles as fluorescent probes in bioassay and fluorescence imaging is feasible.
本文介绍了通过反相微乳液法合成水相CdTe量子点嵌入二氧化硅纳米颗粒及其作为荧光探针在生物测定和细胞成像中的应用。为了在二氧化硅球中嵌入更多的CdTe量子点，我们使用聚（二甲基二烯丙基氯化铵）来平衡CdTe量子点与二氧化硅中间体之间的静电排斥。通过用氨基和甲基膦酸酯基团修饰CdTe/SiO₂复合纳米颗粒的表面，可以获得具有生物功能化且单分散的CdTe/SiO₂复合纳米颗粒。在这项工作中，CdTe/SiO₂复合纳米颗粒通过共价结合与生物素标记的小鼠IgG偶联。CdTe/SiO₂复合纳米颗粒表面的生物素标记的小鼠IgG可以通过蛋白质-蛋白质结合识别聚苯乙烯微球表面的异硫氰酸荧光素标记的抗生物素蛋白和抗生物素蛋白。最后，带有二抗的CdTe/SiO₂复合纳米颗粒成功地用于用一抗标记MG63骨肉瘤细胞，这表明CdTe/SiO₂复合纳米颗粒作为荧光探针在生物测定和荧光成像中的应用是可行的。
