Sathish T, Lakshmi G S, Rao Ch S, Brahmaiah P, Prakasham R S
Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India.
Lett Appl Microbiol. 2008 Oct;47(4):256-62. doi: 10.1111/j.1472-765X.2008.02413.x.
Investigation of mixture-design impact on glutaminase production by isolated Bacillus sp.
An augmented simplex centroid design was used to optimize a three (wheat bran, Bengal gram husk and palm seed fibre) component mixture for glutaminase production. Selected substrate materials showed impact on glutaminase production values at individual level by Bengal gram husk [2789 U gds(-1) (gram dry substrate] and in two-level combination with wheat bran and Bengal gram husk (maximum of 3300 U gds(-1)).
Bengal gram husk is the most suitable substrate medium for glutaminase production by Bacillus sp. Maximum glutaminase production is achieved using solid-substrate mixture at two-level combinations in the ratio of 66 : 34 for Bengal gram husk and wheat bran, respectively.
The present study has significance in large-scale production of glutaminase at commercial level with the use of multi-substrate rather than single-substrate/support material.
研究混合设计对分离出的芽孢杆菌属谷氨酰胺酶产量的影响。
采用扩充单纯形重心设计法优化由三种成分（麦麸、鹰嘴豆壳和棕榈籽纤维）组成的用于谷氨酰胺酶生产的混合物。所选底物材料在个体水平上对谷氨酰胺酶产量有影响，鹰嘴豆壳的产量为[2789 U gds(-1)（克干底物）]，与麦麸和鹰嘴豆壳的二级组合产量最高（达3300 U gds(-1)）。
鹰嘴豆壳是芽孢杆菌属生产谷氨酰胺酶最合适的底物培养基。使用固体底物混合物，鹰嘴豆壳与麦麸比例为66:34的二级组合可实现谷氨酰胺酶的最高产量。
本研究对于在商业层面利用多底物而非单一底物/支撑材料大规模生产谷氨酰胺酶具有重要意义。
