改良风疹血凝抑制试验：用磷脂酶C使非免疫球蛋白血凝抑制剂失活

Improved rubella hemagglutination inhibtion test: inactivation of non-immunoglobulin hemagglutination inhibitors by phospholipase C.

作者信息

Iwasa S, Hori M

出版信息

J Clin Microbiol. 1976 Dec;4(6):461-6. doi: 10.1128/jcm.4.6.461-466.1976.

DOI:10.1128/jcm.4.6.461-466.1976

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274505/

Abstract

A method using phospholipase C (PL-C) for removing nonspecific inhibitors (NSI) of rubella virus hemagglutinin is described. PL-C was found to hydrolyze NSI without altering the hemagglutination inhibition (HI) activity of the specific antibody and could be used to remove NSI in the rubella HI test by using formalinized erythrocytes, which resisted the enzymatic action; fresh erythrocytes were lysed by PL-C. The HI test using PL-C treated sera gave true measurements of actual rubella antibody content, and HI titers of PL-C treated sera were identical or equivalent (+/-1 dilution) to those of sera treated with dextran sulfate and CaCl2 (DS-C). Thus, the PL-C method gave results as reproducible and reliable as the DS-C method and was more convenient.

摘要

描述了一种使用磷脂酶C（PL-C）去除风疹病毒血凝素非特异性抑制剂（NSI）的方法。发现PL-C可水解NSI，而不改变特异性抗体的血凝抑制（HI）活性，并且可通过使用抗酶作用的甲醛化红细胞用于去除风疹HI试验中的NSI；新鲜红细胞会被PL-C裂解。使用PL-C处理血清的HI试验给出了实际风疹抗体含量的真实测量值，并且PL-C处理血清的HI效价与用硫酸葡聚糖和氯化钙（DS-C）处理的血清相同或等效（±1个稀释度）。因此，PL-C方法给出的结果与DS-C方法一样可重复且可靠，并且更方便。

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Biochem J. 1948;42(4):587-90.

2

The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins.细菌毒素的生物化学：产气荚膜梭菌毒素的卵磷脂酶活性。

Biochem J. 1941 Sep;35(8-9):884-902. doi: 10.1042/bj0350884.

3

INHIBITORS OF ECHOVIRUS AND REOVIRUS HEMAGGLUTINATION. II. SERUM AND PHOSPHOLIPID INHIBITORS.艾柯病毒和呼肠孤病毒血细胞凝集抑制剂。II. 血清和磷脂抑制剂。

J Immunol. 1964 Sep;93:377-86.

4

Application of a microtechnique to viral serological investigations.一种微技术在病毒血清学研究中的应用。

J Immunol. 1962 Mar;88:320-9.

5

Human gamma globulin fractionation on anion exchange cellulose columns.人γ-球蛋白在阴离子交换纤维素柱上的分级分离。

J Biol Chem. 1959 Oct;234:2645-51.

6

One-step growth curve of Western equine encephalomyelitis virus on chicken embryo cells grown in vitro and analysis of virus yields from single cells.西部马脑炎病毒在体外培养的鸡胚细胞上的一步生长曲线及单细胞病毒产量分析

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7

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