Campbell J B, Grunberger T, Ellins M L
Can J Microbiol. 1975 Aug;21(8):1172-7. doi: 10.1139/m75-175.
The HEPES-saline-albumin-gelatin (HSAG) diluent found optimal for agglutination of fowl erythrocytes by rubella virus antigen is also optimal for agglutination of trypsin-treated human group O cells. Albumins from different commercial sources, however, can have varying inhibitory effects on rubella hemagglutination titers. This can have a significant effect on the hemagglutination-inhibition test since antibody titers measured by this procedure are related to the amount of antigen used.
已发现对风疹病毒抗原凝集禽红细胞最为适宜的HEPES-盐水-白蛋白-明胶(HSAG)稀释剂,对胰蛋白酶处理过的人O型细胞的凝集也最为适宜。然而,不同商业来源的白蛋白对风疹血凝滴度可能有不同的抑制作用。这可能会对血凝抑制试验产生重大影响,因为通过该程序测得的抗体滴度与所用抗原的量有关。