Kobayashi H, Hayashi M, Kobayashi S, Kabuto M, Handa Y, Kawano H, Ide H
Department of Neurosurgery, Fukui Medical School, Japan.
Neurosurgery. 1991 May;28(5):673-8; discussion 678-9. doi: 10.1097/00006123-199105000-00006.
We investigated the histological changes between arteries constricted by endothelin for 7 days and vasospastic arteries induced by the double injection of autologous blood. Group 1 was a sham-operated group. Group 2 animals received a continuous cisternal injection of endothelin-1 (1.7 x 10(-9) mol/7 days) by a miniosmotic pump implanted in the neck musculature for 7 days. Group 3 received double injections of cisternal blood administered 48 hours apart. Angiography showed severe constriction of the basilar artery, 34.6% and 43% in Groups 2 and 3, respectively, on Day 7. Histological study showed marked constriction of the basilar artery in both Group 2 and Group 3. Degenerative changes in endothelial cells and smooth muscle cells were observed in both Group 2 and Group 3. Immunohistochemical study demonstrated endothelin-1 in the endothelial cells in Group 2, but not in Group 1 or in Group 3. It is suggested that endothelin-1 may play an important role in the pathogenesis of cerebral vasospasm.
我们研究了用内皮素收缩7天的动脉与通过两次注射自体血诱导的血管痉挛性动脉之间的组织学变化。第1组为假手术组。第2组动物通过植入颈部肌肉组织的微型渗透泵持续脑池内注射内皮素-1（1.7×10⁻⁹摩尔/7天），持续7天。第3组接受间隔48小时的两次脑池内血液注射。血管造影显示，在第7天，第2组和第3组基底动脉严重收缩，分别为34.6%和43%。组织学研究显示第2组和第3组基底动脉均有明显收缩。在第2组和第3组均观察到内皮细胞和平滑肌细胞的退行性变化。免疫组织化学研究显示第2组内皮细胞中有内皮素-1，而第1组和第3组中没有。提示内皮素-1可能在脑血管痉挛的发病机制中起重要作用。
