Ceviker N, Baykaner M K, Keskil I S, Sencer H, Ataoģlu O, Ozsoy M, Ercan Z S
Gazi University Faculty of Medicine, Department of Neurosurgery, Ankara, Turkey.
Acta Neurochir (Wien). 1995;132(1-3):104-9. doi: 10.1007/BF01404856.
In this study intra-arterial Endothelin-1 was applied to rabbit basilar arteries and morphological findings were compared between two groups who were either perfusion fixed or immersion fixed. We planned to establish the quantitative dimension of the drug-induced morphological alterations, independent of the fixation technique's effect. There was an abundance of collagenous fibres deposited among the smooth muscle cells which was not observed in control arteries and after immersion fixation. These degenerative changes are similar to the finding following subarachnoid haemorrhage. The only fixation-related difference was the fact that lamina elastica interna was not corrugated in the perfusion fixation group. It is concluded that, the observed changes in the connective tissue of the arterial wall alter the passive elastic properties and so affect the degree of the response to the vasoactive messengers.
在本研究中，将动脉内注射内皮素-1应用于兔基底动脉，并比较了灌注固定组和浸泡固定组的形态学结果。我们计划确定药物诱导的形态学改变的定量维度，而不受固定技术的影响。在平滑肌细胞之间有大量胶原纤维沉积，这在对照动脉和浸泡固定后未观察到。这些退行性变化与蛛网膜下腔出血后的发现相似。唯一与固定相关的差异是，灌注固定组的内弹性膜没有形成皱襞。得出的结论是，观察到的动脉壁结缔组织变化改变了被动弹性特性，从而影响了对血管活性信使的反应程度。
