灵芝深层培养中添加油脂对菌丝体和多糖产量影响的实验分析

Experimental analysis of the oil addition effect on mycelia and polysaccharide productions in Ganoderma lucidum submerged culture.

作者信息

Huang Hung-Chang, Chen Chih-I, Hung Chia-Nan, Liu Yung-Chuan

机构信息

Department of Chemical Engineering, National Chung Hsing University, 250, Kuo Kuang Road, Taichung 40227, Taiwan, ROC.

出版信息

Bioprocess Biosyst Eng. 2009 Feb;32(2):217-24. doi: 10.1007/s00449-008-0239-z. Epub 2008 Sep 2.

DOI:10.1007/s00449-008-0239-z

PMID:18762989

Abstract

The effect of corn oil addition on mycelium growth and polysaccharide productions in the medicinal mushroom Ganoderma lucidum was studied. The results showed that when a level of 2% corn oil was added at the beginning of culture, the biomass and polysaccharide productions reached a maximum of 12.9 and 1.038 g/L, respectively, during 13-day cultivation. The pH variation along with morphology observation in culture provided an indirect inference to the promotional effect of oil addition. Moreover, a curve fitting analysis was carried out to assay the elevated effect on biomass and exopolysaccharide productions in oil added culture. The experimental data of substrates consumption and products formation in culture with oil addition were predicted through the fitting equations obtained in single carbon source culture. The numerical results further clarified the stimulatory effects of oil addition in G. lucidum culture.

摘要

研究了添加玉米油对药用真菌灵芝菌丝体生长和多糖产生的影响。结果表明，在培养开始时添加2%的玉米油，在13天的培养过程中，生物量和多糖产量分别达到最大值，即12.9 g/L和1.038 g/L。培养过程中的pH变化以及形态观察为添加油的促进作用提供了间接推断。此外，进行了曲线拟合分析，以测定添加油的培养物中生物量和胞外多糖产量的提高效果。通过在单一碳源培养中获得的拟合方程预测了添加油的培养物中底物消耗和产物形成的实验数据。数值结果进一步阐明了添加油对灵芝培养的刺激作用。