Kaur Manpreet, Sharma Sandeep, Kaur Gurcharan
Department of Biotechnology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India.
Biogerontology. 2008 Dec;9(6):441-54. doi: 10.1007/s10522-008-9168-0. Epub 2008 Sep 2.
Recent studies on the effects of dietary restriction (DR) in rodents and primates have shown that even late-onset short-term regimens can bring about comparable beneficial changes seen in animals subjected to life-long DR. We studied the effect of aging on the expression of neural cell adhesion molecule (NCAM), its polysialylated form PSA-NCAM and astrocytic marker glial fibrillary acidic protein (GFAP) by immunohistofluorescent staining and immunoblotting in 1, 3, 6, 18 and 24 months old male wistar rats. Maximum expression of NCAM and PSA-NCAM was observed in sub-granular zone (SGZ) or granular cell layer (GCL) of hippocampus, arcuate region and paraventricular area of hypothalamus and piriform cortex layer II from 1 and 3 months old rats, thereafter, gradual downregulation was observed in 6, 18 and 24 months old rats. Progressive increase in astrocytic GFAP expression was noticed in these regions of brain with age. We further addressed whether DR initiated in late adulthood in 24 months old rats confers beneficial effects and can reverse changes in expression of NCAM, PSA-NCAM and GFAP. These results suggest that even late-onset short term DR regimen in old rats can have beneficial effects on neuroplasticity.
近期对啮齿动物和灵长类动物进行的饮食限制（DR）效应研究表明，即使是晚期开始的短期方案也能带来与长期接受饮食限制的动物所见相当的有益变化。我们通过免疫荧光染色和免疫印迹法，研究了1、3、6、18和24月龄雄性Wistar大鼠衰老对神经细胞黏附分子（NCAM）、其多唾液酸化形式PSA-NCAM和星形胶质细胞标志物胶质纤维酸性蛋白（GFAP）表达的影响。在1和3月龄大鼠的海马颗粒下区（SGZ）或颗粒细胞层（GCL）、下丘脑弓状区域和室旁区域以及梨状皮层II层中观察到NCAM和PSA-NCAM的最大表达，此后，在6、18和24月龄大鼠中观察到逐渐下调。随着年龄增长，在这些脑区中星形胶质细胞GFAP表达逐渐增加。我们进一步探讨了在24月龄大鼠成年晚期开始的饮食限制是否具有有益作用，以及是否能逆转NCAM、PSA-NCAM和GFAP表达的变化。这些结果表明，即使是老年大鼠晚期开始的短期饮食限制方案也可能对神经可塑性产生有益影响。
