使用带紫外检测器的高效液相色谱法同时检测丹参注射液中的七种酚酸。
Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector.
作者信息
Xu Jin-zhong, Shen Jie, Cheng Yi-yu, Qu Hai-bin
机构信息
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
出版信息
J Zhejiang Univ Sci B. 2008 Sep;9(9):728-33. doi: 10.1631/jzus.B0820095.
Abstract
A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection. According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A. The limit of detection (LOD) was improved to be in the range of 0.008-0.160 microg/ml. Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes.
摘要
已开发出一种带有紫外（UV）检测器的高效液相色谱（HPLC）方法，用于同时定量测定丹参注射液中的丹酚酸、原儿茶醛、咖啡酸、丹酚酸D、迷迭香酸、丹酚酸B和丹酚酸A。根据这些成分的紫外光谱，选择了三个检测波长，如下所示：丹酚酸和原儿茶醛为280nm，咖啡酸、丹酚酸D和迷迭香酸为326nm，丹酚酸B和丹酚酸A为286nm。检测限（LOD）提高到0.008 - 0.160μg/ml范围内。此外，在所研究的浓度范围内观察到了良好的线性行为，所有分析物的R>0.999。
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