Zhong Guo-Xin, Li Peng, Zeng Ling-Jie, Guan Jia, Li De-Qiang, Li Shao-Ping
Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
J Agric Food Chem. 2009 Aug 12;57(15):6879-87. doi: 10.1021/jf901462s.
The chemical characteristics of Salvia miltiorrhiza , also called "danshen" in China, were investigated on the basis of the simultaneous quantitative determination of 13 hydrophilic and lipophilic compounds, namely, protocatechuic acid, protocatechuic aldehyde, caffeic acid, ferulic acid, isoferulic acid, rosmarinic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, przewalskin, cryptotanshinone, tanshinone I, and tanshinone IIA, in 74 samples collected from different locations using ultraperformance liquid chromatography (UPLC). Hierarchical clustering analysis based on 13 investigated compounds and the similarity of the entire chromatographic pattern showed that S. miltiorrhiza was significantly different from Salvia przewalskii , an adulterant of danshen. The chemical characteristics of S. miltiorrhiza collected from different locations in China were revealed, and salvianolic acid B, rosmarinic acid, cryptotanshinone, and tanshinones I and IIA were optimized as markers for the evaluation, which is helpful in the quality control of S. miltiorrhiza.
基于对74个采自不同地点的样品中13种亲水性和脂溶性化合物（即原儿茶酸、原儿茶醛、咖啡酸、阿魏酸、异阿魏酸、迷迭香酸、丹酚酸B、丹酚酸A、二氢丹参酮I、紫丹参甲素、隐丹参酮、丹参酮I和丹参酮IIA）的同时定量测定，对中国名为“丹参”的丹参的化学特征进行了研究，采用超高效液相色谱（UPLC）进行分析。基于13种被测化合物和整个色谱图的相似性进行的层次聚类分析表明，丹参与丹参的掺假品甘西鼠尾草有显著差异。揭示了采自中国不同地点的丹参的化学特征，并优化了丹酚酸B、迷迭香酸、隐丹参酮以及丹参酮I和IIA作为评价指标，这有助于丹参的质量控制。
