Asgatay Saâdia, Petermann Christian, Harakat Dominique, Guillaume Dominique, Taylor John-Stephen, Clivio Pascale
Université de Reims Champagne Ardenne, Institut de Chimie Moléculaire de Reims, CNRS UMR 6229, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims Cedex, France.
J Am Chem Soc. 2008 Sep 24;130(38):12618-9. doi: 10.1021/ja805214s. Epub 2008 Sep 3.
Using the analogue of TpT methylated at the 3'-end N3 position (Tpm3T), we demonstrate that when the oxetane/(6-4) pathway is precluded, water addition occurs at the 3'-end C6 position of the oxetane intermediate to provide its opening. Photoreversal of this (6-4) photoproduct C6 hydrate brings the first experimental evidence that the (6-4) photolyase repair can proceed through an oxetane intermediate.
