血清中微小RNA的特征：用于癌症和其他疾病诊断的一类新型生物标志物。
Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases.
作者信息
Chen Xi, Ba Yi, Ma Lijia, Cai Xing, Yin Yuan, Wang Kehui, Guo Jigang, Zhang Yujing, Chen Jiangning, Guo Xing, Li Qibin, Li Xiaoying, Wang Wenjing, Zhang Yan, Wang Jin, Jiang Xueyuan, Xiang Yang, Xu Chen, Zheng Pingping, Zhang Juanbin, Li Ruiqiang, Zhang Hongjie, Shang Xiaobin, Gong Ting, Ning Guang, Wang Jun, Zen Ke, Zhang Junfeng, Zhang Chen-Yu
机构信息
Jiangsu Diabetes Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, China.
出版信息
Cell Res. 2008 Oct;18(10):997-1006. doi: 10.1038/cr.2008.282.
Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.
多种组织中微小RNA（miRNA）表达失调与包括癌症在内的多种疾病相关。在此我们证明，miRNA存在于人类以及小鼠、大鼠、牛胎儿、小牛和马等其他动物的血清和血浆中。血清中miRNA的水平在同一物种的个体间是稳定、可重复且一致的。我们利用Solexa技术对健康中国受试者的所有血清miRNA进行了测序，分别在男性和女性受试者中发现了100多种和91种以上的血清miRNA。我们还确定了肺癌、结直肠癌和糖尿病患者血清miRNA的特定表达模式，为血清miRNA包含各种疾病的特征提供了证据。通过定量逆转录聚合酶链反应分析，在75名健康供体和152名癌症患者的独立试验中进一步验证了通过Solexa技术获得的两种非小细胞肺癌特异性血清miRNA。通过这些分析，我们得出结论，血清miRNA可作为检测各种癌症和其他疾病的潜在生物标志物。
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