Oleĭnik A G, Skurikhina L A
Genetika. 2008 Jul;44(7):885-95.
RFLP analysis of three amplified mtDNA fragments (Cytb/D-loop, ND1/ND2, and ND3/ND4L/ND4) was performed in the following taxa: Parahucho perryi, Hucho taimen, Brachymystax lenok, B. tumensis, Salmo salar, Salvelinus leucomaenis, and S. levanidovi. For mtDNA of P. perryi, a substantial decrease in the haplotype and nucleotide diversity was observed as a result of random genetic drift, caused by a reduction in the effective population size. Nucleotide divergence estimates between the mtDNA haplotypes were determined. Sakhalin taimen P. perryi was found to be approximately equally diverged from S. salar and from the charrs of the genus Salvelinus, by 11.0 and 10.0%, respectively. The divergence between P. perryi and H. taimen constituted 14.6%, between P. perryi and lenoks of the genus Brachymystax, 14.2%, and between H. taimen and Brachymystax, 7.7%. The analysis of possible phylogenetic relationships of the mtDNA from P. perryi among the group of taxa examined confirmed validity of the genus Parahucho. Phylogenetic reconstructions performed showed that robustness of the trees constructed for the complex of phylogenetically informative characters over three mtDNA fragments was considerably higher than that of the trees constructed for individual genes.
对以下分类群的三个扩增线粒体DNA片段（细胞色素b/控制区、ND1/ND2和ND3/ND4L/ND4）进行了限制性片段长度多态性（RFLP）分析：石川哲罗鱼、哲罗鲑、细鳞鲑、图们江细鳞鲑、大西洋鲑、白斑红点鲑和利氏红点鲑。对于石川哲罗鱼的线粒体DNA，由于有效种群数量减少导致随机遗传漂变，观察到单倍型和核苷酸多样性大幅下降。确定了线粒体DNA单倍型之间的核苷酸分歧估计值。发现萨哈林哲罗鱼与大西洋鲑以及红点鲑属的红点鲑的分歧程度大致相同，分别为11.0%和10.0%。石川哲罗鱼与哲罗鲑之间的分歧为14.6%，与细鳞鲑属的细鳞鲑之间为14.2%，哲罗鲑与细鳞鲑之间为7.7%。对石川哲罗鱼线粒体DNA在所研究分类群中的可能系统发育关系进行的分析证实了石川哲罗鱼属的有效性。进行的系统发育重建表明，基于三个线粒体DNA片段构建的系统发育信息特征复合体的树的稳健性明显高于基于单个基因构建的树。
