Gallardo-Moreno Amparo M, Pacha-Olivenza Miguel A, Saldaña Laura, Pérez-Giraldo Ciro, Bruque José M, Vilaboa Nuria, González-Martín M Luisa
Department of Applied Physics, Faculty of Science, University of Extremadura, Avda. Elvas s/n, 06071 Badajoz, Spain.
Acta Biomater. 2009 Jan;5(1):181-92. doi: 10.1016/j.actbio.2008.07.028. Epub 2008 Aug 6.
UV irradiation leads to a "spontaneous" wettability increase of the Ti6Al4V surface while preserving bulk properties of the alloy that are crucial for its performance as an orthopedic and dental implant. We hypothesized that UV treatment of Ti6Al4V may impair bacterial adhesion without compromising the good response of human bone-forming cells to this alloy. The in vitro biocompatibility of the Ti6Al4V surface, before and after UV irradiation, was analyzed by using human cells related to the osteoblastic phenotype. The adhesion processes of bacterial strains related to clinical orthopedic infections, i.e., Staphylococcus aureus and Staphylococcus epidermidis, were studied theoretically and in vitro, under dynamic and static conditions as well as in the presence or absence of shear forces. While human cell adhesion was not altered by UV irradiation of Ti6Al4V alloy, this treatment reduced not only initial bacterial adhesion rates but also the number of bacteria retained on the surface after the passage of two air-liquid interfaces on the previously adhered bacteria. This study proposes the use of UV treatment prior to implantation protocols as an easy, economic and effective way of reducing bacterial adhesion on the Ti6Al4V surface without compromising its excellent biocompatibility.
紫外线照射会使Ti6Al4V表面的润湿性“自发”增加，同时保持该合金的整体性能，这些性能对于其作为骨科和牙科植入物的性能至关重要。我们假设，对Ti6Al4V进行紫外线处理可能会损害细菌黏附，同时又不会影响人骨形成细胞对该合金的良好反应。通过使用与成骨细胞表型相关的人类细胞，分析了紫外线照射前后Ti6Al4V表面的体外生物相容性。在动态和静态条件下以及在有或没有剪切力的情况下，从理论和体外研究了与临床骨科感染相关的细菌菌株，即金黄色葡萄球菌和表皮葡萄球菌的黏附过程。虽然Ti6Al4V合金的紫外线照射不会改变人类细胞的黏附，但这种处理不仅降低了初始细菌黏附率，还减少了先前黏附的细菌经过两个气液界面后留在表面的细菌数量。本研究提出在植入方案之前使用紫外线处理，作为一种简单、经济且有效的方法来减少Ti6Al4V表面的细菌黏附，同时又不损害其优异的生物相容性。
