Oleshko Oleksandr, Liubchak Iryna, Husak Yevheniia, Korniienko Viktoriia, Yusupova Aziza, Oleshko Tetiana, Banasiuk Rafal, Szkodo Marek, Matros-Taranets Igor, Kazek-Kęsik Alicja, Simka Wojciech, Pogorielov Maksym
Biomedical Research Centre, Sumy State University, 40018 Sumy, Ukraine.
NanoWave, 02-676 Warsaw, Poland.
Materials (Basel). 2020 Sep 30;13(19):4359. doi: 10.3390/ma13194359.
Despite the high biocompatibility and clinical effectiveness of Ti-based implants, surface functionalization (with complex osteointegrative/antibacterial strategies) is still required. To enhance the dental implant surface and to provide additional osteoinductive and antibacterial properties, plasma electrolytic oxidation of a pure Ti was performed using a nitrilotriacetic acid (NTA)-based Ag nanoparticles (AgNP)-loaded calcium-phosphate solution. Chemical and structural properties of the surface-modified titanium were assessed using scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) and contact angle measurement. A bacterial adhesion test and cell culture biocompatibility with collagen production were performed to evaluate biological effectiveness of the Ti after the plasma electrolytic process. The NTA-based calcium-phosphate solution with Ag nanoparticles (AgNPs) can provide formation of a thick, porous plasma electrolytic oxidation (PEO) layer enriched in silver oxide. Voltage elevation leads to increased porosity and a hydrophilic nature of the newly formed ceramic coating. The silver-enriched PEO layer exhibits an effective antibacterial effect with high biocompatibility and increased collagen production that could be an effective complex strategy for dental and orthopedic implant development.
尽管钛基植入物具有高生物相容性和临床有效性，但仍需要进行表面功能化处理（采用复杂的骨整合/抗菌策略）。为了改善牙科植入物表面并提供额外的骨诱导和抗菌性能，使用基于次氮基三乙酸（NTA）的负载银纳米颗粒（AgNP）的磷酸钙溶液对纯钛进行了等离子体电解氧化处理。使用带有能量色散X射线（EDX）的扫描电子显微镜（SEM）和接触角测量来评估表面改性钛的化学和结构性质。进行了细菌粘附试验以及与胶原蛋白产生相关的细胞培养生物相容性试验，以评估等离子体电解处理后钛的生物学有效性。含有银纳米颗粒（AgNP）的基于NTA的磷酸钙溶液能够形成富含氧化银的厚的多孔等离子体电解氧化（PEO）层。电压升高会导致新形成的陶瓷涂层孔隙率增加和亲水性增强。富含银的PEO层具有有效的抗菌作用，同时具有高生物相容性且能增加胶原蛋白的产生，这可能是一种用于牙科和骨科植入物开发的有效复合策略。
