Rouse Susan, Canchaya Carlos, van Sinderen Douwe
Department of Microbiology, National University of Ireland, Western Road, Cork, Ireland.
Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2013-7. doi: 10.1099/ijs.0.65584-0.
A high-throughput screening effort, designed to isolate bacteriocin-producing lactic acid bacteria (LAB) from malted cereals, resulted in the isolation of four bacteriocin-producing strains that could not be assigned conclusively to recognized species. The four isolates (UCC128T, UCC125, UCC126 and UCC127) were found to share identical (100%) 16S rRNA gene sequences and were therefore deemed to belong to the same species. The strains were Gram-positive, catalase-negative, non-motile homofermentative LAB. The closest recognized relative to strain UCC128T identified based on comparative 16S rRNA gene sequence analysis was Lactobacillus mali DSM 20444T (97% similarity). The strains were characterized phenotypically to identify specific growth requirements. DNA-DNA hybridization between strain UCC128T and L. mali DSM 20444T revealed a level of relatedness of only 39.4%. This indicates that strain UCC128T does not belong to the species L. mali. The four bacteriocin-producing strains are therefore considered to represent a novel species of the genus Lactobacillus, for which the name Lactobacillus hordei sp. nov. is proposed. The type strain is UCC128T (=DSM 19519T=LMG 24241T).
一项旨在从发芽谷物中分离产细菌素乳酸菌（LAB）的高通量筛选工作，分离出了4株产细菌素的菌株，这些菌株无法明确归类到已知的物种。发现这4株分离株（UCC128T、UCC125、UCC126和UCC127）的16S rRNA基因序列完全相同（100%），因此被认为属于同一物种。这些菌株为革兰氏阳性、过氧化氢酶阴性、不运动的同型发酵乳酸菌。基于比较16S rRNA基因序列分析，与菌株UCC128T最接近的已知亲缘种是苹果乳酸杆菌DSM 20444T（相似度97%）。对这些菌株进行了表型特征分析以确定其特定的生长需求。菌株UCC128T与苹果乳酸杆菌DSM 20444T之间的DNA-DNA杂交显示亲缘关系水平仅为39.4%。这表明菌株UCC128T不属于苹果乳酸杆菌物种。因此，这4株产细菌素的菌株被认为代表了乳酸菌属的一个新物种，为此提出名称为麦芽乳酸杆菌（Lactobacillus hordei sp. nov.）。模式菌株是UCC128T（=DSM 19519T=LMG 24241T）。
