Mañes-Lázaro Rosario, Song Jaeho, Pardo Isabel, Cho Jang-Cheon, Ferrer Sergi
ENOLAB-Laboratorio de Microbiología Enológica, Departamento de Microbiología y Ecología, Facultad de Biología, Universidad de Valencia, Dr Moliner 50, 46100 Burjasot, Valencia, Spain.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2215-8. doi: 10.1099/ijs.0.008276-0. Epub 2009 Jul 20.
A Lactobacillus strain, IMCC1736T, was isolated recently from a Korean freshwater pond following an extensive study of the microbial community in this ecosystem. Its 16S rRNA gene was sequenced and phylogenetic analysis placed this strain within the Lactobacillus salivarius group, closely related to Lactobacillus satsumensis NRIC 0604T, with 97.9% sequence similarity. In the present work, the taxonomic status of strain IMCC1736T has been re-evaluated. It was characterized phylogenetically, genotypically and phenotypically and, based on DNA-DNA hybridization values, this strain represents a novel Lactobacillus species. Strain IMCC1736T can be differentiated genotypically from its closest relatives by randomly amplified polymorphic DNA analysis and ribotyping patterns; phenotypically, it can be distinguished by its inability to grow in 5% NaCl and at pH 3.3 and by certain carbohydrate fermentations. Strain IMCC1736T is Gram-positive, catalase-negative and microaerophilic. Cells are motile rods and show homofermentative metabolism. The name Lactobacillus aquaticus sp. nov. is proposed, with strain IMCC1736T (=CECT 7355T=DSM 21051T) as the type strain.
最近，在对韩国一个淡水池塘生态系统中的微生物群落进行广泛研究后，分离出了一株乳酸杆菌菌株IMCC1736T。对其16S rRNA基因进行了测序，系统发育分析将该菌株归入唾液乳杆菌群，与萨摩乳杆菌NRIC 0604T密切相关，序列相似性为97.9%。在本研究中，对菌株IMCC1736T的分类地位进行了重新评估。通过系统发育、基因型和表型特征分析，并基于DNA-DNA杂交值，该菌株代表了一个新的乳酸杆菌物种。菌株IMCC1736T可通过随机扩增多态性DNA分析和核糖体分型模式在基因型上与其最接近的亲缘种进行区分；在表型上，它不能在5% NaCl和pH 3.3条件下生长以及某些碳水化合物发酵情况可将其区分开来。菌株IMCC1736T革兰氏阳性，过氧化氢酶阴性，微需氧。细胞为运动性杆状，呈现同型发酵代谢。提议将其命名为水生乳酸杆菌新种，菌株IMCC1736T（=CECT 7355T=DSM 21051T）作为模式菌株。
