Yoon Jung-Hoon, Kang So-Jung, Im Wan-Taek, Lee Sung-Taik, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusong, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2224-8. doi: 10.1099/ijs.0.65291-0.
A Gram-negative, non-spore-forming, rod-shaped bacterial strain, K106(T), was isolated from wastewater collected from a textile dye works in Korea. Strain K106(T) grew optimally at pH 7.0-7.5 and 30-37 degrees C in the presence of 0-1.0% (w/v) NaCl. A phylogenetic tree based on 16S rRNA gene sequences showed that strain K106(T) joined the type strain of Chelatococcus asaccharovorans with a bootstrap resampling value of 99.9%. The predominant ubiquinone of strain K106(T) was Q-10. The fatty acid profile of strain K106(T) was similar to that of C. asaccharovorans DSM 6462(T). Major polar lipids of strain K106(T) and C. asaccharovorans DSM 6462(T) were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two aminolipids and two unidentified phospholipids. sym-Homospermidine, spermidine and putrescine were major polyamines. The DNA G+C content was 68.3 mol%. Strain K106(T) exhibited 16S rRNA gene sequence similarity of 96.6% to the type strain of C. asaccharovorans. DNA-DNA relatedness data and differential phenotypic properties, particularly differences in cell morphology and the ability to utilize nitrilotriacetate, demonstrated that strain K106(T) can be differentiated from C. asaccharovorans. On the basis of phenotypic, phylogenetic and genetic data, strain K106(T) represents a novel species of the genus Chelatococcus, for which the name Chelatococcus daeguensis sp. nov. is proposed. The type strain is K106(T) (=KCTC 12979(T) =CCUG 54519(T)).
从韩国一家纺织染料厂采集的废水中分离出一株革兰氏阴性、不形成芽孢、杆状的细菌菌株K106(T)。菌株K106(T)在pH 7.0 - 7.5和30 - 37℃、0 - 1.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的系统发育树表明，菌株K106(T)与嗜糖螯合球菌模式菌株聚在一起，自展重抽样值为99.9%。菌株K106(T)的主要泛醌为Q - 10。菌株K106(T)的脂肪酸谱与嗜糖螯合球菌DSM 6462(T)相似。菌株K106(T)和嗜糖螯合球菌DSM 6462(T)的主要极性脂为磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、两种氨基脂和两种未鉴定的磷脂。对称高亚精胺、亚精胺和腐胺是主要的多胺。DNA G + C含量为68.3 mol%。菌株K106(T)与嗜糖螯合球菌模式菌株的16S rRNA基因序列相似性为96. .6%。DNA - DNA相关性数据和不同的表型特征，特别是细胞形态和利用次氮基三乙酸能力的差异，表明菌株K106(T)可与嗜糖螯合球菌区分开来。基于表型、系统发育和遗传数据，菌株K106(T)代表螯合球菌属的一个新物种，为此提出大邱螯合球菌（Chelatococcus daeguensis）新种。模式菌株为K106(T)（=KCTC 12979(T) =CCUG 54519(T)）。
