Chen Yi-Guang, Tang Shu-Kun, Zhang Yu-Qin, Li Zhao-Yang, Yi Lang-Bo, Wang Yong-Xia, Li Wen-Jun, Cui Xiao-Long
Key Laboratory for Conservation and Utilization of Plant Resources of Hunan Province, College of Biology and Environmental Sciences, Jishou University, Jishou, People's Republic of China.
Antonie Van Leeuwenhoek. 2009 Jun;96(1):63-70. doi: 10.1007/s10482-009-9336-5. Epub 2009 Apr 1.
A novel Gram-positive, halotolerant, non-sporulating, non-motile, catalase-positive, oxidase-negative and aerobic bacterium, designated strain JSM 078085(T), was isolated from sea water collected from the South China Sea. Strain JSM 078085(T) exhibited a rod-coccus growth cycle and produced a yellow pigment. The strain was able to grow in the presence of 0-12% (w/v) NaCl and at pH 6.0-9.5 and 4-35 degrees C; optimum growth was observed at pH 7.0 and 25-30 degrees C in the absence of NaCl. The peptidoglycan type was A4alpha (L: -Lys-L: -Ala-L: -Glu). Cell-wall sugars contained galactose and glucose. Strain JSM 078085(T) contained menaquinone MK-9(H(2)) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The major cellular fatty acids were anteiso-C(15:0), iso-C(15:0) and anteiso-C(17:0) and the DNA G + C content was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 078085(T) should be assigned to the genus Arthrobacter, being most closely related to the type strain of Arthrobacter rhombi (sequence similarity 97.1%), and the two strains formed a distinct lineage in the phylogenetic tree. The level of DNA-DNA relatedness between strain JSM 078085(T) and the type strain of Arthrobacter rhombi was 10.6%. The combination of phylogenetic analysis, DNA-DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078085(T) represents a novel species of the genus Arthrobacter, for which the name Arthrobacter halodurans sp. nov. is proposed. The type strain is JSM 078085(T) (=DSM 21081(T)=KCTC 19430(T)).
从中国南海采集的海水中分离出一株新型革兰氏阳性、耐盐、不产芽孢、无运动性、过氧化氢酶阳性、氧化酶阴性的需氧细菌，命名为菌株JSM 078085(T)。菌株JSM 078085(T)呈现杆状-球菌生长周期，并产生黄色色素。该菌株能够在0-12%（w/v）NaCl、pH 6.0-9.5和4-35℃条件下生长；在无NaCl时，pH 7.0和25-30℃条件下观察到最佳生长。肽聚糖类型为A4α（L：-Lys-L：-Ala-L：-Glu）。细胞壁糖类包含半乳糖和葡萄糖。菌株JSM 078085(T)含有甲基萘醌MK-9(H2)作为主要呼吸醌，双磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇作为主要极性脂类。主要细胞脂肪酸为anteiso-C15:0、iso-C15:0和anteiso-C17:0，DNA G + C含量为63.3 mol%。基于16S rRNA基因序列比较的系统发育分析表明，菌株JSM 078085(T)应归属于节杆菌属，与菱形节杆菌模式菌株关系最为密切（序列相似性97.1%），且这两个菌株在系统发育树中形成一个独特的分支。菌株JSM 078085(T)与菱形节杆菌模式菌株之间的DNA-DNA相关性水平为10.6%。系统发育分析、DNA-DNA相关性、表型特征和化学分类数据的综合结果支持以下观点：菌株JSM 078085(T)代表节杆菌属的一个新物种，为此提出新名称嗜盐节杆菌（Arthrobacter halodurans sp. nov.）。模式菌株为JSM 078085(T)（=DSM 21081(T)=KCTC 19430(T)）。
