National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum, India.
Appl Biochem Biotechnol. 2010 Jan;160(2):421-7. doi: 10.1007/s12010-008-8345-6. Epub 2008 Sep 4.
Solid-state fermentation (SSF) was carried out for the production of extracellular alpha-galactosidase by Streptomyces griseoloalbus. Soybean flour was the best solid substrate for alpha-galactosidase production. Packed-bed bioreactor performed well in enhancing the enzyme yield and resulted in a highest yield of 197.2 +/- 0.02 U/gds with a forced aeration of 2 vvm, which was approximately twofold the yield obtained in flasks. The alpha-galactosidase production was growth-associated, and the highest enzyme yield was obtained after 96 h of incubation. It is significant that this is the first report on alpha-galactosidase production by a filamentous bacterium in SSF using packed-bed bioreactor.
采用固态发酵（SSF）技术，利用灰色链霉菌生产胞外α-半乳糖苷酶。在所有固态基质中，豆粉是最适合生产α-半乳糖苷酶的基质。填充床生物反应器在提高酶产量方面表现出色，在强制通气量为 2 vvm 的条件下，酶的最高产量达到了 197.2±0.02 U/gds，是摇瓶发酵的两倍。α-半乳糖苷酶的生产与菌体生长密切相关，在培养 96 小时后可获得最高的酶产量。值得注意的是，这是首次采用填充床生物反应器在 SSF 中利用丝状细菌生产α-半乳糖苷酶的报道。
