Shankar S K, Mulimani V H
Department of Biochemistry, Gulbarga University, Gulbarga, 585106, Karnataka, India.
Bioresour Technol. 2007 Mar;98(4):958-61. doi: 10.1016/j.biortech.2006.03.013. Epub 2006 May 19.
Comparisons were made for alpha-galactosidase production using red gram plant waste (RGPW) with wheat bran (WB) and other locally available substrates using the fungus Aspergillus oryzae under solid-state fermentation (SSF). RGPW proved to be potential substrate for alpha-galactosidase production as it gave higher enzyme titers (3.4 U/g) compared to WB (2.7 U/g) and other substrates tested. Mixing WB with RGPW (1:1, w/w) resulted enhanced alpha-galactosidase yield. The volume of moistening agent in the ratio of 1:2 (w/v), pH 5.5 and 1 ml (1 x 10(6) spores) of inoculum volume and four days incubation were optimum for alpha-galactosidase production. Increase in substrate concentration (RGPW+WB) did not decrease enzyme yield in trays.
在固态发酵（SSF）条件下，使用米曲霉，比较了红克植物废料（RGPW）与麦麸（WB）及其他当地可得底物用于生产α-半乳糖苷酶的情况。结果表明，RGPW是生产α-半乳糖苷酶的潜在底物，因为与WB（2.7 U/g）及其他测试底物相比，它产生的酶活性更高（3.4 U/g）。将WB与RGPW按1:1（w/w）混合可提高α-半乳糖苷酶的产量。对于α-半乳糖苷酶的生产，润湿剂体积比为1:2（w/v）、pH 5.5、接种量为1 ml（1×10⁶个孢子）以及培养四天是最佳条件。增加底物浓度（RGPW + WB）并不会降低托盘内的酶产量。
