Keller H E
Carl Zeiss, Inc., Thornwood, New York, USA.
Curr Protoc Cytom. 2001 May;Chapter 2:Unit 2.1. doi: 10.1002/0471142956.cy0201s00.
The optical microscope is a fundamental component of an image cytometry system. Electronic imaging has both greatly enhanced the capabilities of light microscopes and placed ever-increasing demands on their optical performance. This unit covers the basic concepts of light microscopy, including Köhler illumination, resolution, contrast, and numerical aperture, and reviews the many types of instruments and techniques for contrast enhancement.
光学显微镜是图像细胞分析系统的基本组成部分。电子成像既极大地增强了光学显微镜的功能，也对其光学性能提出了越来越高的要求。本单元涵盖了光学显微镜的基本概念，包括柯勒照明、分辨率、对比度和数值孔径，并回顾了多种用于增强对比度的仪器和技术。
