Rajwa Bartek
Purdue University, Cytometry Laboratories, West Lafayette, Indiana, USA.
Curr Protoc Cytom. 2005 Feb;Chapter 12:Unit 12.3. doi: 10.1002/0471142956.cy1203s31.
This unit re-examines confocal microscopy from a current perspective. It outlines many of the most modern applications of confocal microscopy and the issues surrounding them. The expanding applications of confocal microscopy demand both minor and major modifications of the technology to enhance imaging capabilities for a growing variety of samples. Techniques of interest such as FRET, FLIP, FRAP, and IFRAP are described. The unit includes a discussion on multispectral imaging, potentially the most exciting innovation in the field of biological imaging. The other area that is beginning to have considerable impact on biology, medicine, and pharmacology comprises high-content screening (HCS) and high-throughput screening (HTS). This unit also introduces programmable array microscopes (PAMs), which are based upon a technique in which spatial modulators are placed in the imaging plane of a microscope and used to generate patterns of illumination and/or detection.
本单元从当前视角重新审视共聚焦显微镜。它概述了共聚焦显微镜的许多最现代应用以及与之相关的问题。共聚焦显微镜不断扩展的应用要求对该技术进行大大小小的改进，以增强对越来越多样的样本的成像能力。文中描述了诸如荧光共振能量转移（FRET）、荧光损失在光漂白（FLIP）、荧光漂白恢复（FRAP）和红外荧光漂白恢复（IFRAP）等相关技术。本单元还讨论了多光谱成像，这可能是生物成像领域最令人兴奋的创新。另一个开始对生物学、医学和药理学产生重大影响的领域包括高内涵筛选（HCS）和高通量筛选（HTS）。本单元还介绍了可编程阵列显微镜（PAM），它基于一种技术，即将空间调制器放置在显微镜的成像平面中，并用于生成照明和/或检测模式。
