• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用JC-1对线粒体膜电位进行流式细胞术分析。

Flow cytometric analysis of mitochondrial membrane potential using JC-1.

作者信息

Cossarizza A, Salvioli S

机构信息

University of Modena, Modena, Italy.

出版信息

Curr Protoc Cytom. 2001 May;Chapter 9:Unit 9.14. doi: 10.1002/0471142956.cy0914s13.

DOI:10.1002/0471142956.cy0914s13
PMID:18770751
Abstract

Changes in membrane potential have long been known to represent early activation events. This unit presents very recent developments in both fluorescent probes and functional applications and demonstrates the use of the JC-1 probe for measuring mitochondrial membrane potential by flow cytometry. A valuable component of this measurement system is the possibility of making quantitative measurements of changes in membrane potential.

摘要

长期以来，人们一直知道膜电位的变化代表早期激活事件。本单元介绍了荧光探针和功能应用方面的最新进展，并展示了使用JC-1探针通过流式细胞术测量线粒体膜电位的方法。该测量系统的一个重要组成部分是能够对膜电位的变化进行定量测量。

相似文献

1
Flow cytometric analysis of mitochondrial membrane potential using JC-1.使用JC-1对线粒体膜电位进行流式细胞术分析。
Curr Protoc Cytom. 2001 May;Chapter 9:Unit 9.14. doi: 10.1002/0471142956.cy0914s13.
2
Sensitive and reliable JC-1 and TOTO-3 double staining to assess mitochondrial transmembrane potential and plasma membrane integrity: interest for cell death investigations.用于评估线粒体跨膜电位和质膜完整性的灵敏且可靠的JC-1和TOTO-3双重染色：对细胞死亡研究的意义
Cytometry A. 2003 Aug;54(2):100-8. doi: 10.1002/cyto.a.10059.
3
Comparison of JC-1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study.JC-1 和 MitoTracker 探针在储存犬血小板浓缩物中线粒体活力评估中的比较：流式细胞术研究。
Cytometry A. 2019 Feb;95(2):214-218. doi: 10.1002/cyto.a.23567. Epub 2018 Aug 14.
4
Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) by using fluorescence-activated flow cytometry.使用荧光激活流式细胞术测定加载线粒体探针5,5',6,6'-四氯-1,1',3,3'-四乙基苯并咪唑基羰花青碘化物（JC-1）的精子中的高线粒体膜电位。
Methods Mol Biol. 2008;477:89-97. doi: 10.1007/978-1-60327-517-0_8.
5
Comparative evaluation of hypoxic-ischemic brain injury by flow cytometric analysis of mitochondrial membrane potential with JC-1 in neonatal rats.应用 JC-1 流式细胞术检测线粒体膜电位对新生大鼠缺氧缺血性脑损伤的比较评价。
J Neurosci Methods. 2010 Nov 30;193(2):232-8. doi: 10.1016/j.jneumeth.2010.08.020. Epub 2010 Sep 15.
6
Multiparameter analysis of physiological changes in apoptosis.细胞凋亡中生理变化的多参数分析
Curr Protoc Cytom. 2001 May;Chapter 9:Unit 9.15. doi: 10.1002/0471142956.cy0915s14.
7
Flow cytometric chemosensitivity assay using JC‑1, a sensor of mitochondrial transmembrane potential, in acute leukemia.应用 JC-1 检测急性白血病线粒体跨膜电位的流式细胞术化疗药敏试验
Cancer Chemother Pharmacol. 2013 Dec;72(6):1335-42. doi: 10.1007/s00280-013-2303-x.
8
Uncompensated Polychromatic Analysis of Mitochondrial Membrane Potential Using JC-1 and Multilaser Excitation.使用JC-1和多激光激发对线粒体膜电位进行无补偿多色分析。
Curr Protoc Cytom. 2015 Apr 1;72:7.32.1-7.32.11. doi: 10.1002/0471142956.cy0732s72.
9
Visna/maedi virus-induced apoptosis involves the intrinsic mitochondrial pathway.维斯纳/梅迪病毒诱导的细胞凋亡涉及内源性线粒体途径。
Arch Virol. 2004 Jul;149(7):1293-307. doi: 10.1007/s00705-004-0302-2. Epub 2004 Mar 25.
10
Labeling mitochondria with JC-1.用JC-1标记线粒体。
Cold Spring Harb Protoc. 2011 Sep 1;2011(9):pdb.prot065490. doi: 10.1101/pdb.prot065490.

引用本文的文献

1
ABT-263, a BCL-2 inhibitor, selectively eliminates latently HIV-1-infected cells without viral reactivation.ABT-263，一种BCL-2抑制剂，可在不激活病毒的情况下选择性清除潜伏感染HIV-1的细胞。
PLoS One. 2025 May 20;20(5):e0322962. doi: 10.1371/journal.pone.0322962. eCollection 2025.
2
In vitro investigation of the toxicological mechanisms of Fingolimod (S)-phosphate in HEPG2 cells.芬戈莫德（S）-磷酸盐在HEPG2细胞中毒理学机制的体外研究
Toxicol Res (Camb). 2025 May 4;14(3):tfaf064. doi: 10.1093/toxres/tfaf064. eCollection 2025 Jun.
3
Isolated Mitochondrial Preparations and Assays: A Powerful and Relevant Tool for Assessment of Brain (Patho)physiology.
分离的线粒体制剂和测定法：评估脑（病理）生理学的有力且相关工具。
Curr Neuropharmacol. 2023;21(6):1433-1449. doi: 10.2174/1570159X21666230303123555.
4
Biodegradable nanoparticulate co-delivery of flavonoid and doxorubicin: Mechanistic exploration and evaluation of anticancer effect and .类黄酮与阿霉素的可生物降解纳米颗粒共递送：抗癌作用的机制探索与评估
Biomater Biosyst. 2021 Jul 30;3:100022. doi: 10.1016/j.bbiosy.2021.100022. eCollection 2021 Sep.
5
Targeted-Lymphoma Drug Delivery System Based on the Sgc8-c Aptamer.基于Sgc8-c适配体的靶向淋巴瘤药物递送系统
Cancers (Basel). 2023 Feb 1;15(3):922. doi: 10.3390/cancers15030922.
6
The Effects of Resveratrol-Rich Extracts of Pruning Waste on HeLa, MCF-7 and MRC-5 Cells: Apoptosis, Autophagia and Necrosis Interplay.修剪废弃物中富含白藜芦醇的提取物对HeLa、MCF-7和MRC-5细胞的影响：细胞凋亡、自噬与坏死的相互作用
Pharmaceutics. 2022 Sep 23;14(10):2017. doi: 10.3390/pharmaceutics14102017.
7
Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock.在复苏的猪内毒素血症休克模型中，单核细胞亚群激活的特定隔室差异不受一氧化氮和糖皮质激素治疗的影响。
J Clin Med. 2022 May 8;11(9):2641. doi: 10.3390/jcm11092641.
8
Optimization of PhysicoChemical Parameters for Production of Cytotoxic Secondary Metabolites and Apoptosis Induction Activities in the Culture Extract of a Marine Algal-Derived Endophytic Fungus sp.海洋藻类内生真菌sp.培养提取物中细胞毒性次生代谢产物生产及凋亡诱导活性的物理化学参数优化
Front Pharmacol. 2021 Apr 26;12:542891. doi: 10.3389/fphar.2021.542891. eCollection 2021.
9
The Potential Use of Sialic Acid From Edible Bird's Nest to Attenuate Mitochondrial Dysfunction by Study.食用燕窝中唾液酸通过研究减轻线粒体功能障碍的潜在用途。
Front Pharmacol. 2021 Apr 12;12:633303. doi: 10.3389/fphar.2021.633303. eCollection 2021.
10
Polyhexamethylene Guanidine Phosphate Induces Apoptosis through Endoplasmic Reticulum Stress in Lung Epithelial Cells.聚六亚甲基胍磷酸盐通过内质网应激诱导肺上皮细胞凋亡。
Int J Mol Sci. 2021 Jan 26;22(3):1215. doi: 10.3390/ijms22031215.