Model Michael A
Kent State University, Kent, Ohio, USA.
Curr Protoc Cytom. 2006 Aug;Chapter 10:Unit10.14. doi: 10.1002/0471142956.cy1014s37.
Standardization in image cytometry involves intensity calibration and shading correction. This unit presents a method using concentrated solutions of fluorophores. A drop of highly concentrated dye solution placed between a slide and a coverslip produces a spatially uniform fluorescent sample with reproducible quantum yield and resistance to photobleaching. The technique has a number of practical features that make it inexpensive, reproducible, and straightforward. Descriptions are given for both wide-field and confocal scanning fluorescence microscopy.
图像细胞术的标准化涉及强度校准和阴影校正。本单元介绍了一种使用荧光团浓缩溶液的方法。一滴高浓度染料溶液置于载玻片和盖玻片之间，可产生具有可重复量子产率且抗光漂白的空间均匀荧光样本。该技术具有许多实用特性，使其成本低廉、可重复且操作简便。文中给出了宽场和共聚焦扫描荧光显微镜的相关描述。
