Phelan Mary C
Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.
Curr Protoc Cytom. 2006 May;Appendix 3:Appendix 3B. doi: 10.1002/0471142956.cya03bs36.
This unit opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
本单元开篇详细讨论了无菌技术的最新原则和培养基的制备。分步方案描述了胰蛋白酶消化和单层培养物的传代培养、悬浮培养物的传代、细胞的冻融、使用血细胞计数器计数细胞以及准备运输的细胞。
