Techniques for mammalian cell tissue culture.
作者信息
Phelan Mary C
机构信息
Molecular Pathology Laboratory Network, Maryville, Tennessee.
出版信息
Curr Protoc Protein Sci. 2006 Dec;Appendix 3:A.3C.1-A.3C.18. doi: 10.1002/0471140864.psa03cs46.
PMID:18429293
Abstract
This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
摘要
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