[鬼臼毒素对人胃癌细胞系SGC 7901的作用]
[Effect of podophyllotoxin on human gastric cancer cell line SGC 7901].
作者信息
Zhang Jie, Zhou Chun-shan, Liu Shao, Chen Hao, Yang Chao
机构信息
Department of Pharmacy, Third Xiangya Hospital, College of Chemistry and Chemical Engieering, Changsha 410083, Central South University, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Aug;33(8):718-22.
OBJECTIVE
To investigate the effect of podophyllotoxin on human gastric cancer cell line SGC 7901 cells in vitro and in vivo, and to provide evidence for treatment of gastric cancer with podophyllotoxin.
METHODS
SGC 7901 cells were treated with podophyllotoxin at various concentrations for different time. MTT assay, colony formation assay, and flow cytometry were performed to determine the effect of podophyllotoxin on the growth and apoptosis of SGC 7901 cells in vitro. Furthermore, xenograft experiment was performed to detect the effect of podophyllotoxin on the in vivo tumorigenicity of SGC 7901 cells.
RESULTS
Podophyllotoxin inhibited the growth of SGC 7901 cells in a concentration- and time-dependent manner, decreased the colony formation rate of the cells, induced SGC 7901 cell apoptosis, arrested SGC 7901 cells at G2/M phases of cell cycle, and decreased the volume and weight of SGC 7901 cell xenografts in nude mice.
CONCLUSION
Podophyllotoxin inhibits the growth of human gastric cancer SGC 7901 cells, induce the apoptosis of SGC 7901 cells in vitro, and inhibit the in vivo tumorigenicity of SGC 7901 cells, which provides evidence for treatment of gastric cancer using podophyllotoxin.
目的
研究鬼臼毒素对人胃癌细胞系SGC 7901细胞的体内外作用，为鬼臼毒素治疗胃癌提供依据。
方法
用不同浓度的鬼臼毒素处理SGC 7901细胞不同时间。采用MTT法、集落形成试验和流式细胞术检测鬼臼毒素对SGC 7901细胞体外生长和凋亡的影响。此外，进行异种移植实验以检测鬼臼毒素对SGC 7901细胞体内致瘤性的影响。
结果
鬼臼毒素以浓度和时间依赖的方式抑制SGC 7901细胞的生长，降低细胞的集落形成率，诱导SGC 7901细胞凋亡，使SGC 7901细胞停滞于细胞周期的G2/M期，并减小裸鼠体内SGC 7901细胞异种移植物的体积和重量。
结论
鬼臼毒素抑制人胃癌SGC 7901细胞的生长，诱导SGC 7901细胞体外凋亡，并抑制SGC 7901细胞的体内致瘤性，为利用鬼臼毒素治疗胃癌提供了依据。
Zotero 插件