Wan Fu-sheng, Wu Jian, Li Hua, Tu Shuo, Yu Le-han
Department of Biochemistry and Molecular Biology, Medical College of Nanchang University, Nanchang 330006, China.
Zhong Yao Cai. 2009 Feb;32(2):245-9.
To investigate the effects of extracts of Solanum lyratum (ESL) on the apoptosis of Human stomach cancer SGC-7901 cells.
Dried whole herbs of Solanum lyratum were extracted by boiling distilled water. SGC-7901 cells were randomly divided into control group, ESL-treated groups (12.5 g/L, 25 g/L, 50 g/L) and the positive control (25 mg/L DDP) group. The growth inhibitory rate was evaluated by MTT assay. Morphological changes of apoptosis were observed with fluorescence microscope. Cell apoptosis rate was determined by flow cytometry. Expressions of bcl-xl, Caspase-9 and bid mRNA were detected by semi-quantitive RT-PCR. The activity of Caspase-3 was detected by Fluorospectrophotometry.
Compared with control group, the cell proliferation inhibitory rate and apoptosis rate of human stomach cancer SGC-7901 cells increased obviously (P < 0.05). There were obvious changes of morphology of the SGC-7901 cells as the nuclear shrinkage, chromatin condensation and margination; The expression of bcl-xl mRNA decreased obviously (P < 0.05), the expression of Caspase-9 and bid mRNA increased obviously respectively (P < 0.05), and displayed effect in a dose-dependent manner in the SGC-7901 cells of the ESL-treated groups. The activity of Caspase-3 in the SGC-7901 cells of the ESL-treated groups were higher than that of the control group significantly (P < 0.01).
ESL can induce apoptosis and inhibit proliferation of the human stomach cancer SGC-7901 cells by regulating expression of bcl-xl, Caspase-9 and bid genes and strengthening the activity of Caspase-3.
探讨白毛藤提取物（ESL）对人胃癌SGC - 7901细胞凋亡的影响。
采用蒸馏水煮沸法提取白毛藤全草干燥药材。将SGC - 7901细胞随机分为对照组、ESL处理组（12.5 g/L、25 g/L、50 g/L）和阳性对照组（25 mg/L顺铂）。采用MTT法评估生长抑制率。用荧光显微镜观察凋亡的形态学变化。通过流式细胞术测定细胞凋亡率。采用半定量RT - PCR检测bcl - xl、Caspase - 9和bid mRNA的表达。用荧光分光光度法检测Caspase - 3的活性。
与对照组相比，人胃癌SGC - 7901细胞的增殖抑制率和凋亡率明显增加（P < 0.05）。SGC - 7901细胞出现明显形态学变化，如细胞核缩小、染色质凝聚和边缘化；bcl - xl mRNA表达明显降低（P < 0.05），Caspase - 9和bid mRNA表达分别明显增加（P < 0.05），且在ESL处理组的SGC - 7901细胞中呈剂量依赖性。ESL处理组SGC - 7901细胞中Caspase - 3的活性明显高于对照组（P < 0.01）。
ESL可通过调节bcl - xl、Caspase - 9和bid基因的表达及增强Caspase - 3的活性诱导人胃癌SGC - 7901细胞凋亡并抑制其增殖。
