Wouters M, Vandekerckhove J
J Gen Virol. 1976 Dec;33(3):529-33. doi: 10.1099/0022-1317-33-3-529.
Poliovirus was treated with fluorescamine and disrupted with sodium dodecyl sulphate (SDS). The polypeptides were isolated by electrophoresis in a polyacrylamide gel (SDS-PAGE) and their amino acid compositions determined. The compositions of VP1, 2 and 3 were very similar but different from that of VP4.
脊髓灰质炎病毒用荧光胺处理,并用十二烷基硫酸钠(SDS)裂解。通过聚丙烯酰胺凝胶电泳(SDS-PAGE)分离多肽,并测定其氨基酸组成。VP1、2和3的组成非常相似,但与VP4不同。