Spidlen Josef, Leif Robert C, Moore Wayne, Roederer Mario, Brinkman Ryan R
Terry Fox Laboratory, BC Cancer Agency, Vancouver, British Columbia, Canada.
Cytometry A. 2008 Dec;73A(12):1151-7. doi: 10.1002/cyto.a.20637.
The lack of software interoperability with respect to gating due to lack of a standardized mechanism for data exchange has traditionally been a bottleneck, preventing reproducibility of flow cytometry (FCM) data analysis and the usage of multiple analytical tools. To facilitate interoperability among FCM data analysis tools, members of the International Society for the Advancement of Cytometry (ISAC) Data Standards Task Force (DSTF) have developed an XML-based mechanism to formally describe gates (Gating-ML). Gating-ML, an open specification for encoding gating, data transformations and compensation, has been adopted by the ISAC DSTF as a Candidate Recommendation. Gating-ML can facilitate exchange of gating descriptions the same way that FCS facilitated for exchange of raw FCM data. Its adoption will open new collaborative opportunities as well as possibilities for advanced analyses and methods development. The ISAC DSTF is satisfied that the standard addresses the requirements for a gating exchange standard.
由于缺乏用于数据交换的标准化机制，在门控方面软件缺乏互操作性一直是一个传统瓶颈，这阻碍了流式细胞术（FCM）数据分析的可重复性以及多种分析工具的使用。为促进FCM数据分析工具之间的互操作性，国际细胞计量学促进协会（ISAC）数据标准特别工作组（DSTF）的成员开发了一种基于XML的机制来正式描述门控（门控标记语言）。门控标记语言是一种用于编码门控、数据转换和补偿的开放规范，已被ISAC DSTF采纳为候选推荐标准。门控标记语言能够像FCS促进原始FCM数据交换那样，促进门控描述的交换。它的采用将开启新的合作机会以及进行高级分析和方法开发的可能性。ISAC DSTF确信该标准满足门控交换标准的要求。
