Martin S M, Byers T J
J Protozool. 1976 Nov;23(4):608-13. doi: 10.1111/j.1550-7408.1976.tb03851.x.
The activity and sedimentation of acid phosphatase (APase), acid deoxyribonuclease (DNase), and acid ribonuclease (RNase) were investigated throughout growth and encystment in Acanthamoeba castellanii. The activities/mg protein of all 3 hydrolases are high in young cultures and decrease to constant levels in postlog cells. The RNase activity/ameba decreases 50% during growth, whereas the activity/cell of both APase and DNase remains constant. The percent sedimentation at 20,000 g of all 3 enzymes gradually increases from about 40% in midlog to a plateau of 80% in postlog cells. During encystment, the sedimentation behavior of RNase differs from that of APase and DNase. Encystment is characterized by a differential decrease in the activity/cell of the 3 hydrolases, with RNase decreasing most rapidly and APase least rapidly. APase is unique in that a transient increase of its specific activity is noted during encystment, even though its activity/cell is decreasing.
在卡氏棘阿米巴整个生长和包囊形成过程中，对酸性磷酸酶（APase）、酸性脱氧核糖核酸酶（DNase）和酸性核糖核酸酶（RNase）的活性及沉降情况进行了研究。在年轻培养物中，所有这3种水解酶的活性/毫克蛋白含量都很高，而在对数生长期后的细胞中则降至恒定水平。在生长过程中，RNase活性/阿米巴下降了50%，而APase和DNase的活性/细胞则保持恒定。在20,000 g离心力下，所有这3种酶的沉降百分比从中对数期的约40%逐渐增加到对数生长期后细胞中的80%的平稳期。在包囊形成过程中，RNase的沉降行为与APase和DNase不同。包囊形成的特点是这3种水解酶的活性/细胞呈差异性下降，其中RNase下降最快，APase下降最慢。APase的独特之处在于，尽管其活性/细胞在下降，但在包囊形成过程中其比活性会出现短暂增加。
