van Asbeck E C, Hoepelman A I M, Scharringa J, Verhoef J
Eijkman-Winkler Institute for Medical & Clinical Microbiology, Utrecht University Hospital, The Netherlands.
Int J Antimicrob Agents. 2009 Jan;33(1):21-6. doi: 10.1016/j.ijantimicag.2008.06.013. Epub 2008 Sep 6.
Since caspofungin inhibits fungal cell wall beta-glucan synthesis and the fungal cell wall plays an important role in the recognition of Candida by phagocytic cells, we studied phagocytosis in the presence of caspofungin. The aim of this work was to investigate the effect of pre-treatment of Candida parapsilosis with caspofungin on phagocytic mechanisms (opsonisation, oxidative burst, phagocytosis and killing). C. parapsilosis grown in the presence of caspofungin at concentrations above the minimal inhibitory concentration (MIC) were more difficult to opsonise and to phagocytose. C. parapsilosis exposed to any concentration of caspofungin below and above the MIC was more difficult to kill. Caspofungin-treated C. parapsilosis impaired the oxidative burst. Overall, it appears that caspofungin treatment of C. parapsilosis alters the capacity of polymorphonuclear leukocytes to phagocytose and delays killing of the organism. This may allow C. parapsilosis to persist in tissues.
由于卡泊芬净抑制真菌细胞壁β-葡聚糖的合成，且真菌细胞壁在吞噬细胞识别念珠菌的过程中发挥重要作用，因此我们研究了在卡泊芬净存在的情况下的吞噬作用。这项工作的目的是研究用卡泊芬净预处理近平滑念珠菌对吞噬机制（调理作用、氧化爆发、吞噬作用和杀伤作用）的影响。在高于最低抑菌浓度（MIC）的卡泊芬净浓度下生长的近平滑念珠菌更难被调理和吞噬。暴露于低于和高于MIC的任何浓度卡泊芬净的近平滑念珠菌更难被杀死。经卡泊芬净处理的近平滑念珠菌会损害氧化爆发。总体而言，似乎用卡泊芬净处理近平滑念珠菌会改变多形核白细胞的吞噬能力，并延迟对该生物体的杀伤。这可能使近平滑念珠菌在组织中持续存在。
