Correa José Antonio González, López-Villodres Juan Antonio, Asensi Rocío, Espartero José Luis, Rodríguez-Gutiérez Guillermo, De La Cruz José Pedro
Department of Pharmacology, Laboratorio de Investigaciones Antitrombóticas e Isquemia Tisular (LIAIT), School of Medicine, University of Málaga, Campus de Teatinos s/n, 29071 Málaga, Spain.
Br J Nutr. 2009 Apr;101(8):1157-64. doi: 10.1017/S0007114508061539. Epub 2008 Sep 8.
Hydroxytyrosol acetate (HT-AC) is a polyphenol present in virgin olive oil (VOO) at a proportion similar to hydroxytyrosol (HT) (160-479 micromol/kg oil). The present study was designed to measure the in vitro platelet antiaggregating activity of HT-AC in human whole blood, and compare this effect with that of HT and acetylsalicylic acid (ASA). The experiments were designed according to the standard procedure to investigate the activity of ASA. HT-AC and HT inhibited platelet aggregation induced by ADP, collagen or arachidonic acid in both whole blood and platelet-rich plasma (PRP). ASA and HT-AC had a greater effect in whole blood than in PRP when ADP or collagen was used as inducer. ASA and HT-AC had a greater effect in PRP+leucocytes than in PRP alone. All three compounds inhibited platelet thromboxane B2 and leucocyte 6-keto-prostaglandin F1alpha (6-keto-PF1 alpha) production. The thromboxane/6-keto-PGF1alpha inhibition ratio (as an indirect index of the prostanoid equilibrium) was 10.8 (SE 1) for HT-AC, 1.0 (SE 0.1) for HT and 3.3 (SE 0.2) for ASA. All three compounds stimulated nitric oxide production, although HT was a weaker effect. In our experiments only concentrations higher than 500 microm (HT) or 1 mm (HT-AC and ASA) inhibited 3-nitrotyrosine production. All three compounds inhibited the production of TNFalpha by leucocytes, with no significant differences between them. In quantitative terms HT-AC showed a greater antiplatelet aggregating activity than HT and a similar activity to that of ASA. This effect involved a decrease in platelet thromboxane synthesis and an increase in leucocyte nitric oxide production.
羟基酪醇乙酸酯(HT-AC)是一种存在于初榨橄榄油(VOO)中的多酚,其含量与羟基酪醇(HT)相似(160-479微摩尔/千克油)。本研究旨在测定HT-AC在人全血中的体外血小板抗聚集活性,并将其与HT和乙酰水杨酸(ASA)的作用效果进行比较。实验按照研究ASA活性的标准程序设计。HT-AC和HT在全血和富血小板血浆(PRP)中均能抑制由二磷酸腺苷(ADP)、胶原蛋白或花生四烯酸诱导的血小板聚集。当以ADP或胶原蛋白作为诱导剂时,ASA和HT-AC在全血中的作用效果比在PRP中更显著。与单独的PRP相比,ASA和HT-AC在PRP+白细胞中的作用效果更显著。这三种化合物均能抑制血小板血栓素B2和白细胞6-酮-前列腺素F1α(6-酮-PF1α)的生成。HT-AC的血栓素/6-酮-PGF1α抑制率(作为类前列腺素平衡的间接指标)为10.8(标准误1),HT为1.0(标准误0.1),ASA为3.3(标准误0.2)。这三种化合物均能刺激一氧化氮的生成,不过HT的作用较弱。在我们的实验中,只有高于500微摩尔(HT)或1毫摩尔(HT-AC和ASA)的浓度才能抑制3-硝基酪氨酸的生成。这三种化合物均能抑制白细胞产生肿瘤坏死因子α(TNFα),它们之间没有显著差异。从数量上看,HT-AC的抗血小板聚集活性比HT更强,与ASA的活性相似。这种作用涉及血小板血栓素合成的减少和白细胞一氧化氮生成的增加。