荧光配体作为RNA结构的分子探针。

Fluorescent ligand as a molecular probe for the RNA structure.

作者信息

Umemoto Shiori, Zhang Jinhua, Dohno Chikara, Nakatani Kazuhiko

机构信息

The institute of Scientific and Industrial Reserch, Osaka University, Mihogaoka 8-1, Ibaraki 567-0047, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):211-2. doi: 10.1093/nass/nrn107.

DOI:10.1093/nass/nrn107

PMID:18776328

Abstract

In this report, we show the recognition of RNA secondary structure by exploiting a fluorescent 2,7-disubstituted 9H-xanthen-9-one derivative (2). The xanthone derivertive (2) especially bound to RNA and became non-fluorescent. Upon mixing with RRE model RNA, U-bulge RNA, or hairpin RNA, the fluorescence intensity of 2 was decreased in different intensity. The distinct fluorescent increase of 2 to each RNA seems to a good probe for The RNA structures.

摘要

在本报告中，我们展示了通过利用一种荧光2,7 - 二取代的9H - 呫吨 - 9 - 酮衍生物（2）来识别RNA二级结构。呫吨酮衍生物（2）特别与RNA结合并变得无荧光。与RRE模型RNA、U - 凸起RNA或发夹RNA混合后，2的荧光强度以不同强度降低。2对每种RNA明显的荧光增强似乎是RNA结构的良好探针。