一种开发基于小分子的传感器的策略，该传感器在与特定RNA结合时会强烈荧光。

A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA.

作者信息

Sparano Brian A, Koide Kazunori

机构信息

Department of Chemistry, University of Pittsburgh, Pennsylvania 15260, USA.

出版信息

J Am Chem Soc. 2005 Nov 2;127(43):14954-5. doi: 10.1021/ja0530319.

DOI:10.1021/ja0530319

PMID:16248596

Abstract

We report a broadly applicable approach for the development of small-molecule-based RNA sensors. Our photoinduced electron transfer (PET) sensor consists of a fluorescein derivative as the fluorophore and two aniline derivatives as electron donors (quenchers). The isolation of electron-donor-binding RNA by in vitro selection (also known as SELEX) yielded an RNA aptamer that could increase the fluorescence intensity of the sensor by 13-fold. This result shows that RNA-electron-donor interactions can be used to develop modular RNA chemosensors.

摘要

我们报告了一种广泛适用的基于小分子的RNA传感器开发方法。我们的光诱导电子转移（PET）传感器由一种荧光素衍生物作为荧光团和两种苯胺衍生物作为电子供体（猝灭剂）组成。通过体外筛选（也称为SELEX）分离出与电子供体结合的RNA，得到了一种RNA适配体，它可以使传感器的荧光强度增加13倍。这一结果表明，RNA-电子供体相互作用可用于开发模块化RNA化学传感器。