带有双膦酸盐修饰的四磷酸5' mRNA帽类似物的合成及生化研究

Synthesis and biochemical studies of tetraphosphate 5' mRNA cap analogs bearing bisphosphonate modification.

作者信息

Rydzik Anna, Zuberek Joanna, Kowalska Joanna, Lukaszewicz Maciej, Darzynkiewicz Edward, Jemielity Jacek

机构信息

Division of Biophysics, Institute of Experimental Physics, University of Warsaw, 02-089 Warsaw, Poland.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):287-8. doi: 10.1093/nass/nrn145.

DOI:10.1093/nass/nrn145

PMID:18776366

Abstract

Dinucleotide cap analogs containing tetraphosphate bridge modified by replacing one of the bridging oxygen atoms with methylene group were synthesized. The analogs have been examined for their stability towards enzymatic hydrolysis by human DcpS and binding affinity for eIF4E. Their inhibitory properties in rabbit reticulocyte lysate (RRL) translational system were studied.

摘要

合成了含有四磷酸桥的二核苷酸帽类似物，其中四磷酸桥中的一个桥连氧原子被亚甲基取代。已检测这些类似物对人DcpS酶促水解的稳定性以及对eIF4E的结合亲和力。研究了它们在兔网织红细胞裂解物（RRL）翻译系统中的抑制特性。