Furukawa Kazuhiro, Abe Hiroshi, Wang Jin, Oki Kazuma, Uda Miwako, Tsuneda Satoshi, Ito Yoshihiro
Nano Medical Engineering Laboratory, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 Japan.
Nucleic Acids Symp Ser (Oxf). 2008(52):353-4. doi: 10.1093/nass/nrn178.
A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10-20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.
开发了一种还原触发的荧光探针，其具有从罗丹明110衍生而来的新型荧光化合物，用于传感寡核苷酸。激活该化合物的化学反应涉及罗丹明衍生物的叠氮基团与还原剂之间的反应，叠氮基团还原后会出现荧光信号。该反应在生物条件下进行，在存在靶DNA或RNA的情况下，10 - 20分钟内产生荧光信号。这些探针已成功应用于溶液中寡核苷酸和细菌细胞内源性RNA的检测。
