含吡咯胞苷 - 鸟嘌呤碱基对的无终淬灭剂分子信标的设计
Design of an ultimate quencher free molecular beacon containing pyrrolocytidine-guanine base pair.
作者信息
Saito Yoshio, Shinohara Yuta, Bag Subhendu Sekhar, Takeuchi Yoshiki, Matsumoto Katsuhiko, Saito Isao
机构信息
Department of Materials Chemistry and Engineering and NEWCAT Institute, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.
出版信息
Nucleic Acids Symp Ser (Oxf). 2008(52):361-2. doi: 10.1093/nass/nrn182.
PMID:18776403
Abstract
A novel quencher free molecular beacon was designed in which fluorophore-labelled pyrrolocytidine was placed away from the stem terminal. This new type of MB was used for the detection of a target DNA with an excellent efficiency.
摘要
设计了一种新型的无淬灭剂分子信标，其中荧光团标记的吡咯胞苷远离茎端。这种新型分子信标用于检测靶DNA，效率极高。
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