用于血红素的RNA适配体的体外筛选

In vitro selection of RNA aptamer to hemin.

作者信息

Liu Mingzhe, Kagahara Takuma, Abe Hiroshi, Ito Yoshihiro

机构信息

Nano Medical Engineering Laboratory, RIKEN-The Institute of Physical and Chemical Research, Wako-shi, Saitama, 351-0198, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):513-4. doi: 10.1093/nass/nrn260.

DOI:10.1093/nass/nrn260

PMID:18776479

Abstract

A new RNA aptamer-binding hemin was synthesized by the in vitro selection (SELEX) method. A pool of 103 bases single strand DNAs containing a randomized sequence of 59 bases was synthesized. The pool was incubated with hemin on hemin-immobilized affinity column. Bound RNAs were eluted off with hemin solution and amplified by PCR. After 3 rounds of selection process, the selected RNAs were cloned and sequenced. Some RNA aptamers, which have affinity to hemin, was selected.

摘要

通过体外筛选（SELEX）方法合成了一种新的与血红素结合的RNA适配体。合成了一个包含59个碱基随机序列的103个碱基单链DNA文库。将该文库与固定在亲和柱上的血红素一起孵育。结合的RNA用血红素溶液洗脱并通过PCR扩增。经过三轮筛选过程后，对筛选出的RNA进行克隆和测序。筛选出了一些对血红素有亲和力的RNA适配体。