血红素结合催化性RNA的体外筛选

In vitro selection of hemin-binding catalytic RNA.

作者信息

Liu Mingzhe, Kagahara Takuma, Abe Hiroshi, Ito Yoshihiro

机构信息

Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-Shi, Saitama 351-0198, Japan.

出版信息

Bioorg Med Chem Lett. 2009 Mar 1;19(5):1484-7. doi: 10.1016/j.bmcl.2009.01.016. Epub 2009 Jan 11.

DOI:10.1016/j.bmcl.2009.01.016

PMID:19179075

Abstract

Catalytic RNAs with peroxidase activity were obtained by the in vitro selection of RNA aptamer-binding hemin. One of the RNA aptamers selected showed binding affinity to hemin with a dissociation constant of 0.8muM and exhibited high peroxidase activity by forming a complex with hemin. The catalytic efficiency of the RNA-hemin complex was 10-fold higher than that of hemin alone.

摘要

通过对结合血红素的RNA适体进行体外筛选，获得了具有过氧化物酶活性的催化RNA。所筛选出的一种RNA适体对血红素具有结合亲和力，解离常数为0.8μM，并通过与血红素形成复合物而表现出高过氧化物酶活性。RNA-血红素复合物的催化效率比单独的血红素高10倍。