将阳离子染料掺入DNA以实现双链体的独特稳定。

Incorporation of cationic dyes into DNA for distinct stabilization of duplex.

作者信息

Kashida Hiromu, Itoh Hidehiro, Fujii Taiga, Asanuma Hiroyuki

机构信息

Graduate School of Engineering, Nagoya University, Furocho, Chikusa, Nagoya 464-8603, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):701-2. doi: 10.1093/nass/nrn354.

DOI:10.1093/nass/nrn354

PMID:18776573

Abstract

In this study, cationic dyes (methylstilbazole) were introduced into ODN. When two complementary ODNs, both of which tethered this dye, were hybridized, the melting temperature drastically increased. The duplex was further stabilized by introducing multiple dyes.

摘要

在本研究中，将阳离子染料（甲基二苯乙烯唑）引入寡脱氧核苷酸（ODN）。当两条都连接有这种染料的互补寡脱氧核苷酸杂交时，解链温度急剧升高。通过引入多种染料，双链进一步稳定。