Scatliff Candice E, Bankovic-Calic Neda, Ogborn Malcolm R, Aukema Harold M
Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.
Nephron Exp Nephrol. 2008;110(2):e44-8. doi: 10.1159/000153244. Epub 2008 Sep 8.
BACKGROUND/AIMS: Several dietary interventions, including those involving conjugated linoleic acid (CLA), slow progression of polycystic kidney disease (PKD) when initiated in the early stages of disease in Han:SPRD-cy rats. However, in humans, kidney disease is often undetected until extensive renal injury has developed. The objective of this study therefore was to determine whether initiating dietary CLA intervention in advanced PKD would slow disease progression.
Adult male Han:SPRD-cy rats with advanced kidney disease were fed diets with or without 1% CLA for 16 weeks. Disease progression was assessed by serum urea, proteinuria, and creatinine clearance, and morphological and immunohistochemical measurements for pathologic change.
Renal injury was lower in the PKD rats given CLA compared to those given the control diet as indicated by a reduction in inflammation (42% less), fibrosis (28% less), oxidative damage (30% less) and proliferating cells (35% less). Diet had no effect on body, kidney, or liver weight, serum urea, serum creatinine, creatinine clearance, proteinuria, or cyst volume.
Late dietary intervention with CLA reduced some disease-associated pathologies, but did not alter renal function in adult Han:SPRD-cy rats. The long-term anti-inflammatory, antioxidant, and antiproliferative benefits of CLA in advanced kidney disease remain to be determined.
背景/目的：多项饮食干预措施，包括那些涉及共轭亚油酸（CLA）的措施，在Han:SPRD-cy大鼠疾病早期开始实施时可减缓多囊肾病（PKD）的进展。然而，在人类中，肾脏疾病往往在广泛的肾损伤出现后才被发现。因此，本研究的目的是确定在晚期PKD中开始饮食CLA干预是否会减缓疾病进展。
将患有晚期肾病的成年雄性Han:SPRD-cy大鼠喂食含或不含1%CLA的饮食16周。通过血清尿素、蛋白尿和肌酐清除率以及病理变化的形态学和免疫组织化学测量来评估疾病进展。
与给予对照饮食的大鼠相比，给予CLA的PKD大鼠肾损伤更低，表现为炎症（减少42%）、纤维化（减少28%）、氧化损伤（减少30%）和增殖细胞（减少35%）减少。饮食对体重、肾脏或肝脏重量、血清尿素、血清肌酐、肌酐清除率、蛋白尿或囊肿体积没有影响。
CLA的晚期饮食干预减少了一些与疾病相关的病理变化，但未改变成年Han:SPRD-cy大鼠的肾功能。CLA在晚期肾病中的长期抗炎、抗氧化和抗增殖益处仍有待确定。
