Lee Man Ryul, Kim Soo Kyoung, Kim Jong Soo, Rhim Si Youn, Kim Kye-Seong
Department of Anatomy and Cell Biology, Hanyang University, College of Medicine, Seoul 133-791, Korea.
Mol Cells. 2008 Dec 31;26(6):621-4.
We previously showed that Asb-4 and Asb-17 is uniquely expressed in developing male germ cells. A recent report showed that Asb-9 is specifically expressed in the kidney and testes; however, detailed expression patterns in developing germ cells have not been shown. Northern blot analysis in various tissues demonstrated that mAsb-9 was strongly expressed in the testes. Expression analysis by RT-PCR and Northern blot in developing mouse testes indicates that mAsb-9 is expressed from the fourth week after birth to adulthood, with the highest expression in round spermatids. Expression sites were further localized by in situ hybridization in the testes. Pachytene spermatocytes and spermatids expressed mAsb-9 but spermatogonia and generated spermatozoa did not. This study reveals that mAsb-9 could be a specific marker of active spermatogenesis and would be useful for studies of male germ cell development.
我们之前表明，Asb-4和Asb-17在发育中的雄性生殖细胞中独特表达。最近的一份报告显示，Asb-9在肾脏和睾丸中特异性表达；然而，其在发育中的生殖细胞中的详细表达模式尚未见报道。对各种组织进行的Northern印迹分析表明，mAsb-9在睾丸中强烈表达。通过RT-PCR和Northern印迹对发育中的小鼠睾丸进行的表达分析表明，mAsb-9从出生后第四周开始表达直至成年，在圆形精子细胞中表达最高。通过睾丸原位杂交进一步定位了表达位点。粗线期精母细胞和精子细胞表达mAsb-9，但精原细胞和生成的精子不表达。这项研究表明，mAsb-9可能是活跃精子发生的特异性标志物，对雄性生殖细胞发育的研究将很有用。
