Smith H G, Meltzer M S, Leonard E J
J Natl Cancer Inst. 1976 Oct;57(4):809-13. doi: 10.1093/jnci/57.4.809.
Line 10 hepatoma cells were incubated with antiserum specific against line 10 cells (RaL10) and then tested for growth in syngeneic Wright strain 2 guinea pigs. Palpable tumors appeared in only 11 of 23 animals inoculated id with 10(5) RaL10-treated tumor cells, compared with an incidence of 21 of 23 for nonimmune rabbit serum (NRS)-treated cells and 23 of 23 for cells treated with syngeneic guinea pig serum. Animals inoculated with RaL10-treated tumor cells did not develop systemic tumor immunity. The long-term survival of guinea pigs receiving RaL10-treated tumor cells iv was 6 of 12 with a dose of 10(5) cells and 8 of 11 with 10(4) cells. None of the animals receiving 10(4) or 10(5) control tumor cells treated with NRS survived. RaL10 antiserum was not toxic to line 10 tumor cells in vitro, but mediated tumor-specific cytolytic reactions in the presence of fresh guinea pig serum or on the addition of peritoneal exudate cells from nonimmunized syngeneic guinea pigs.
将10号肝癌细胞与针对10号细胞的特异性抗血清（RaL10）一起孵育，然后在同基因的赖特2号豚鼠中检测其生长情况。皮下接种10⁵经RaL10处理的肿瘤细胞的23只动物中，只有11只出现了可触及的肿瘤，相比之下，用非免疫兔血清（NRS）处理的细胞的发生率为23只中的21只，用同基因豚鼠血清处理的细胞的发生率为23只中的23只。接种经RaL10处理的肿瘤细胞的动物没有产生全身性肿瘤免疫。静脉注射经RaL10处理的肿瘤细胞的豚鼠，剂量为10⁵个细胞时，12只中有6只长期存活，剂量为10⁴个细胞时，11只中有8只长期存活。接受用NRS处理的10⁴或10⁵个对照肿瘤细胞的动物无一存活。RaL10抗血清在体外对10号肿瘤细胞无毒，但在新鲜豚鼠血清存在下或加入未免疫的同基因豚鼠的腹腔渗出细胞后介导肿瘤特异性细胞溶解反应。
