Chen Xiao-Chun, Bai Jian-Xin, Cao Jia-Ming, Li Zhen-Jiang, Xiong Jian, Zhang Lei, Hong Yuan, Ying Han-Jie
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
Bioresour Technol. 2009 Jan;100(2):919-24. doi: 10.1016/j.biortech.2008.07.062. Epub 2008 Sep 7.
Response surface methodology was employed to optimize medium composition for the production of cyclic adenosine 3',5'-monophosphate (cAMP) with Microbacterium sp. no. 205. A fractional factorial design (2(11-7)) was applied to evaluate the effects of different components in the medium. K(2)HPO(4), MgSO(4) and NaF were found to significantly influence on the cAMP production. The steepest ascent method was used to access the optimal region of the medium composition. The concentrations of the three factors were optimized subsequently using central composite design and response surface methodology. The optimal medium composition to achieve the optimal cAMP production was determined (g/L): K(2)HPO(4), 12.78; MgSO(4), 3.53 and NaF, 0.18. The corresponding cAMP concentration was 8.50 g/L, which was about 1.8-fold increase compared with that using the original medium. Validation experiments were also carried out to prove the adequacy and the accuracy of the model obtained. The cAMP fermentation in 5L fermenter reached 9.87 g/L.
采用响应面法优化嗜甲基菌属205号菌株生产3',5'-环磷酸腺苷（cAMP）的培养基成分。应用部分因子设计（2(11-7)）评估培养基中不同成分的影响。发现磷酸氢二钾、硫酸镁和氟化钠对cAMP的生产有显著影响。采用最速上升法确定培养基成分的最佳区域。随后使用中心复合设计和响应面法对这三个因素的浓度进行优化。确定了实现最佳cAMP产量的最佳培养基成分（g/L）：磷酸氢二钾12.78；硫酸镁3.53；氟化钠0.18。相应的cAMP浓度为8.50 g/L，与使用原始培养基相比提高了约1.8倍。还进行了验证实验以证明所获得模型的充分性和准确性。在5L发酵罐中进行的cAMP发酵达到了9.87 g/L。
