El-Sayed Sayed E, Abdelaziz Neveen A, Ali Amer Al, Alshahrani Mohammad Y, Aboshanab Khaled M, El-Housseiny Ghadir S
Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, Giza 12566, Egypt.
Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, University of Bisha, 255, Al Nakhil, Bisha 67714, Saudi Arabia.
Microorganisms. 2023 Nov 22;11(12):2835. doi: 10.3390/microorganisms11122835.
Fungal infections currently pose a real threat to human lives. In the current study, soil bacterial isolates were screened for the production of antifungal compounds to combat human fungal pathogens. Notably, the bacterial F1 isolate exhibited antimycotic action towards the ATCC 10231 and clinical isolates. By employing phenotypic and molecular techniques, we identified the F1 isolate as the isolate OQ071612. The purified extract showed stability within a pH range of 6-7 and at temperatures of up to 50 °C. It demonstrated potential antifungal activity in the presence of various surfactants, detergents, and enzymes. The purified extract was identified as 6-methoxy-1H-Indole-2-carboxylic acid using advanced spectroscopic techniques. To optimize the antifungal metabolite production, we utilized response surface methodology (RSM) with a face-centered central composite design, considering nutritional and environmental variables. The optimal conditions were as follows: starch (5 g/L), peptone (5 g/L), agitation rate of 150 rpm, pH 6, and 40 °C temperature. A confirmatory experiment validated the accuracy of the optimization process, resulting in an approximately 3.49-fold increase in production. This is the first documented report on the production and characterization of 6-methoxy-1H-Indole-2-carboxylic acid (MICA) antifungal metabolite from
真菌感染目前对人类生命构成了切实威胁。在本研究中，对土壤细菌分离株进行了筛选，以寻找能够产生抗真菌化合物来对抗人类真菌病原体的菌株。值得注意的是，细菌分离株F1对ATCC 10231及临床分离株表现出抗真菌作用。通过表型和分子技术，我们将F1分离株鉴定为分离株OQ071612。纯化后的提取物在pH值6 - 7范围内以及高达50°C的温度下表现出稳定性。在存在各种表面活性剂、洗涤剂和酶的情况下，它展现出潜在的抗真菌活性。使用先进的光谱技术将纯化后的提取物鉴定为6 - 甲氧基 - 1H - 吲哚 - 2 - 羧酸。为了优化抗真菌代谢产物的生产，我们采用了响应面法（RSM）并结合了中心复合设计，同时考虑营养和环境变量。最佳条件如下：淀粉（5 g/L）、蛋白胨（5 g/L）、搅拌速度150 rpm、pH值6以及温度40°C。一项验证性实验证实了优化过程的准确性，产量提高了约3.49倍。这是关于从……生产和表征6 - 甲氧基 - 1H - 吲哚 - 2 - 羧酸（MICA）抗真菌代谢产物的首篇文献报道。
